英语翻译Reality comes furthest from ideal with separations among closely related groups of molecules,such as product variants.Misfields,dimidiated forms,or enzymatic cleavage products may bear such a high degree of homology to the correct product

英语翻译Reality comes furthest from ideal with separations among closely related groups of molecules,such as product variants.Misfields,dimidiated forms,or enzymatic cleavage products may bear such a high degree of homology to the correct product
英语翻译
Reality comes furthest from ideal with separations among closely related groups of molecules,such as product variants.Misfields,dimidiated forms,or enzymatic cleavage products may bear such a high degree of homology to the correct product form that even orthogonal methods may be challenged.It occasionally occurs that a single method will remove one type of product variant with good success but in general,the greater the similarity among the product and contaminants,the more essential orthogonal design becomes for achieving the necessary level of purification.
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These examples emphasize an important feature of orthogonal process design:the purification capability of any one step is measurable only within the context of its partner(s).This is illustrated by comparison of Figures 1 and 2.In Figure 1,each method is capable of achieving 90% purification from raw product,but a particular contaminant co-elutes with the product in both.In Figure 2,the first method is the same as in Figure 1.The second method achieves only 60% purification from raw sample,but when combined with the first,the result is 100% purity because no shared contaminants co-elute with the product.This reveals the inherent fallacy in judging individual purification methods based solely on their average purification factor.Context is paramount.

英语翻译Reality comes furthest from ideal with separations among closely related groups of molecules,such as product variants.Misfields,dimidiated forms,or enzymatic cleavage products may bear such a high degree of homology to the correct product
实体,理论上是由最初理想状态下成组的关系紧密的分子构成的.如:乘积变量, Misfields,二分表. 为了获得正确的产品形式,酶分裂产物也会有高度的同族关系,甚至其垂直路径也会被更改.时而会发生一种单一的方式成功代替一种产品变量的事情,但总体上讲,产品与污染物的成分越类似,垂直结构就会越精良从而达到净化的目的.
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这些例子都强调直角工艺流程的一个重要的特色:任何一个步骤的净化能力都只是又其合伙人的书面报告形式来评价的.又图1和图2的对照可以看出.图1中任何一种方式都可以从原料达到90%的提纯度.但是有种特殊的污染物会同产物一起洗提.图2中,第一种方式与图1相同,第2种方式只能从原料样本中获得60%的提纯度.但是一旦与第一种方式相结合,会产生100%提纯的效果,因为产物中不会有共同污染物与其洗提.这显示对于只是建立在平均提纯因素上的单独提纯方式的评价是固有的谬论.前后环境是十分重要的.
(花了半小时!)